Isolation Of Antibiotic Producing Bacteria From Paddy Soil In Naen, North Central Timor Regency

  • Lukas Pardosi Universitas Timor
  • Adelya Irawan Manalu Faculty of Agriculture, Timor University
Keywords: isolation, soil bacteria, antibiotics

Abstract

Soil is a habitat for small animals and microorganisms. The most abundant microorganisms in soil are bacteria. The soil used in the study was soil obtained from paddy fields in Naen, North Central Timor Regency.The study aimed to characterize the bacterial isolates obtained from the paddy fields which are known to produce antibiotics. Rice soil samples were taken at a depth of about 15 cm from the soil surface and then cultured in Nutrient Agar media with graded dilutions. Characterization was carried out by macroscopic observation, namely direct observation, then a challenge test was carried out against S. aureus bacteria. The results showed that there were 6 bacterial isolates with macroscopic results mostly irregular in shape and 1 isolate fibrous. There were 4 bacterial isolates namely STS1, STS3, STS4, and STS6 which had inhibitory power against the growth of S. aureus.

Downloads

Download data is not yet available.

References

Ambarwati dan Gama, A. 2009. Isolasi Actinomycetes Dari Tanah Sawah Sebagai Penghasil Antibiotik. Jurnal Penelitian Sains & Teknologi, 10 (2): 101 – 111.

Ardiwinata, A. S. 2007. Petunjuk Teknis Analisis Residu Pestisida. Balai Penelitian Lingkungan Pertanian, Pati.

Berdy J. 2012. Thoughts and Facts about Antibiotics: Where We are Now and Where We are Heading. The Journal of Antibiotics 65, 385–395.

Fitriani., Meylina, L dan Rijai, L. 2016. Isolasi dan Karakterisasi Bakteri Penghasil antibiotik Dari Tanah Sawah. Prosiding Seminar Nasional Kefarmasian Ke-4.

Handayany, G.N., Zulfiati. 2020. Isolasi Mikroba Penghasil Antibiotika dari PasirPantai Lemo-Lemo Kabupaten Bulu kamba dalam Menghambat Beberapa Bakteri Patogen. Jurnal Kesehatan Vol 13 No 1 (2020).

Hardjowigeno, S. dan M. L. Rayes. 2005. Tanah Sawah Karakteristik, Kondisi dan Permasalahan Tanah Sawah di Indonesia. Bayumedia Publishing. Malang.

Pujiati. 2014. Isolasi Actinomycetes Dari Tanah Kebun sebagai Bahan Petunjuk Praktikum Mikrobiologi. Jurnal Florea Volume 1 No. 2, Nopember 2014 (42-46).

Ratnakomala, S., Apriliana, P., Fahrurozi., Lisdiyanti, P., dan Kusharyoto,W. 2016. Aktivitas Antibakteri Aktinomisetes Laut Dari PulauEnggano. BeritaBiologi 15(3).

Susilowati DN, RD Hastutidan E Yuniarti. 2007. Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1. 1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407. JurnalAgro Biogen 3(1), 15-23.

Weber T, P Charusanti, EM Musiol-Kroll, X Jiang, Y Tong, HU Kim and SY Lee. 2015. Metabolic Engineer ing of Antibiotic Factories: New Tools for Antibiotic Production in Actinomycetes. Trends in Biotechnology 33 (1), 15-26
Published
2022-07-29
How to Cite
Pardosi, L., & Manalu, A. (2022). Isolation Of Antibiotic Producing Bacteria From Paddy Soil In Naen, North Central Timor Regency. Jurnal Saintek Lahan Kering, 5(1), 5-6. https://doi.org/https://doi.org/10.32938/slk.v5i1.1780
Section
Original research article