In vitro antibacterial activity of the ethanolic extract of Puni (Zingiber zerumbet) from timor island
Keywords:
Antibacterial activity, Extraction, Puni, Wild Ginger, Zingiber zerumbet
Abstract
Puni (Zingiber zerumbet) is a wildly grown plant that used by Timorese people to treat ulcerative lesions in Timor Island. This study aims to determine the antibacterial activity of plants extracts and know the content of active compounds on the most active extracts of plants. Extraction of active compounds was carried out using four solvent ie n-hexane, ethyl acetate, ethanol, and aqueous solvent. Antibacterial activity test was done using the diffusion method of the four crude extract and extract with the highest activity was then used to find MIC value. Analyzed the content of the active compounds using LC-MS. It was found that ethanol extract had the highest antibacterial activity compared to other extracts. The MIC value of ethanol extract against S. aureus, B. Subtilis, E. coli, and P. aeruginosa were respectively determined to be 50, 100, 150, 250 mg mL-1. The result of LC-MS analysis showed that the ethanol extract contained zerumbone and gingerglycolipid B.
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References
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Yob N J, Jofrry S. M, Affandi M M R, Teh L K, Salleh M Z, Zakaria Z A. 2011. Zingiber zerumbet (L.) Smith: A Review of Its Ethnomedicinal, Chemical, and Pharmacological Uses. Doi: 10.1155/2011/543216
Yoshikawa M, Yamaguchi S, Kunimi K, Matsuda H, Okuno Y, Yamahara J, Murakami N. 1994. Stomachic Principles in Ginger III. An Anti-ulcer Principle, 6-Gingesulfonic acid, and Three Monoacyldigalactosylglycerols, Gingerglycolipids A, B, and C, from Zingiberis Rhizoma Originating in Taiwan. Chem Pharm Bull. 42 (6) 1226-1230.
Clinical and Laboratory Standards Institute (CLSI). 2007. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. M100-S17. Vol. 27 No. 1
Cowan M M. 1999. Plant Products as Antimicrobial Agents. Clin. Microbiol. Rev. Vol 12, No. 4
de Fatima A, Modo LV, Conegero LS, Pilli RA, Ferreira CV, Kohn LK, de Carvalho JE. 2006. Lactones and Their Derivatives Biological Activities, Mechanism of Action and Potential Leads for Drugs Design. J. Med. Chem. (13): 3371-3384
Griffin S. 2000. Aspect of Antimicrobial Activity of Terpenoids and The Relationship to Their Molecular Structure [Disertation]. New South Wales (AU): University of Western Sidney
Harborne J B. 1996. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan, Diterjemahkan oleh Kosasih Padmawinata dan Iwang Soediro. Bandung (ID): Penerbit ITB
Kader M G, Nikkon F, Rashid M A, Yeasmin T. 2011. Antimicrobial Activities of The Rhizome Extract of Zingiber zerumbet Linn. Asian Pac J Trop Biomed. 1(5):409-412
Kim SJ, Chang J, Singh M. 2015. Peptidoglycan Architecture of Gram-positive Bacteria by Solid-state NMR. Biochim Biophys Acta. 1848: 350-362. doi: 10.1016/j.bbamem.2014.05.031.
Kitayama T, Yokoi T, Kawai Y, Hill RK, Morita M, Okamoto T, Yamamoto K, Fokin VV, Sharpless KB, Sawada S. 2003. Chemistry of Zerumbone. Part 5: Structural Transformation of The Dimethylamine Derivatives. Tetrahedron. 59: 4857-4866
Kress WJ, Prince LM, Wiliam KJ. 2002. Phylogeny and A New Classification of The Gingers (Zingiberaceae): Evidance from Molecular Data. Am. J. Botany 89 (11): 1682-1696.
Kress WJ, 2014. Zingiberales Research Website. Washington DC, USA: Smithsonian Institution.
Larsen K, Ibrahim H, Khaw SH, Saw LG. 1999. Pollination and Seed Dispersal, p. 19-20. In: K. M. Wong (Ed.). Ginger of Penninsula Malaysia and Singapura. Natural History Publication (Borneo). Kinabalu
Lawalata V N. 2012. Rekayasa Proses Ekstraksi Kulit Buah Langsat (Lansium domesticum var. langsat) sebagai Bahan Antibakteri dan Antioksidan. [disertasi].Bogor (ID): Program pascasarjana, Institut Pertanian Bogor
Nag A, Bandyopadhyay M, Mukherjee A. 2013. Antioxidant Activities and Cytotoxicity of Zingiber zerumbet (L.) Smith Rhizome. J Pharmacogn Phytochem. 2 (3): 102-108
Nagori BP, Solanki R. 2011. Role of Medicinal Plants in Wound Healing. Res. J. Med. Plant. 5 (4): 392-405, 2011
Nursal W, Sri, Wilda S. 2006. Bioaktifitas Ekstrak Jahe (Zingiber officinale Roxb.) dalam Menghambat Pertumbuhan Koloni Bakteri Escherichia coli dan Bacillus subtilis. Jurnal Biogenesis 2(2): 64-66
Parhusip AJN. 2006. Kajian Mekanisme Antibakteri Ekstrak Andaliman (Zanthoxylum acanthopodium DC) Terhadap Bakteri Patogen Pangan [disertasi]. Bogor (ID): Sekolah Pascarjana Institut Pertanian Bogor.
Santosh Kumar SC, Srinivas P, Negi PS, Bettadaiah BK. 2013. Antibacterial and Antimutagenic Activities of Novel Zerumbone Analogues. Food Chem., 141: 1097-1103
Sari KIP, Periadnadi, Nasir N. 2013. Uji Antimikroba Ekstrak Segar Jahe-Jahean (Zingiberaceae) Terhadap S. aureus, E. coli dan C. albicans. J. Bio. UA. 2 (1)-Maret 2013
Selmar D. 2008. Potensial of Salt and Drought Stress to Increase Pharmaceutical Significant Secondary Coumpounds in Plants. Agriculture and forestry research. 58:139-144
Sinaga E, Suprihatin, Wiryanti I. 2011. Perbandingan Daya Sitotoksik Ekstrak Rimpang 3 Jenis Tumbuhan Zingiberaceae Terhadap Sel Kanker MCF-7. Jurnal Farmasi Indonesia Vol. 5 No. 3. Januari 2011: 125-133
Singh CB, Nongalleima Kh, Brojendrosingh S, Ningobam S, Lokendrajit N, Singh LW. 2012 Biological and Chemical Properties of Zingiber zerumbet Smith: A Review. Phytochem Rev. 11:113-125
Skarzynski T, Mistry A, Wonacott A, Hutchinson SE, Kelly VA, Duncan K. 1996. Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, An Enzyme Essential for The Synthesis of Bacterial Peptidoglycan, Complexed with Substrate UDP-N-acetylglucosamine and The Drug Fosfomycin. Structure. 4: 1465-1474.
Sopandie D. 2014. Fisiologi Adaptasi Tanaman Terhadap Cekaman Abiotik pada Agroekositem Tropika. Bogor (ID): IPB Press
Vollmer W, Seligman SJ. 2010. Architecture of Peptidoglycan: More Data and More Models. Trend in Microbiol. Vol 18, No. 2
Voravuthikuncai SP, Limsuwan S, Supapol O, Subhadhirasakul S. 2006. Antibacterial Activity of Extracts from Family Zingiberaceae Against Foodborne Pathogens. J Food Safety. 26: 325-334.
Yasni S. 2013. Teknologi Pengolahan dan Pemanfaatan Produk Ekstraktif Rempah. Bogor: IPB Press.
Yob N J, Jofrry S. M, Affandi M M R, Teh L K, Salleh M Z, Zakaria Z A. 2011. Zingiber zerumbet (L.) Smith: A Review of Its Ethnomedicinal, Chemical, and Pharmacological Uses. Doi: 10.1155/2011/543216
Yoshikawa M, Yamaguchi S, Kunimi K, Matsuda H, Okuno Y, Yamahara J, Murakami N. 1994. Stomachic Principles in Ginger III. An Anti-ulcer Principle, 6-Gingesulfonic acid, and Three Monoacyldigalactosylglycerols, Gingerglycolipids A, B, and C, from Zingiberis Rhizoma Originating in Taiwan. Chem Pharm Bull. 42 (6) 1226-1230.
Published
2017-04-20
How to Cite
Kapitan, O., Ambarsari, L., & Falah, S. (2017). In vitro antibacterial activity of the ethanolic extract of Puni (Zingiber zerumbet) from timor island. Savana Cendana, 2(02), 29-32. https://doi.org/https://doi.org/10.32938/sc.v2i02.82
Section
Original research article
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