Mass Propagation on Dendrobium Orchid ‘Gradita 31’ Through Somatic Embryogenesis
Keywords:
Benzyladenine, Dendrobium, Embryogenesis
Abstract
Dendrobium tissue culture through somatic embryogenesis process is a solution for mass propagation. The concentration of PGR and the composition of basic media are important factors in somatic embryogenesis. This research aimed to obtain the best type of basic media and BA concentration for regeneration process through somatic embryogenesis on Dendrobium 'Gradita 31' orchid. This research used a completely randomized design with 3 replications arranged factorial. The first factor was the type of basic media, ½ MS and MS. The second factor was concentration of cytokinin BA 1, 2 and 3 mg/L combined with NAA 0.5 mg/L. Each experimental unit consisted of 2 culture bottles, each contained 2 explants. The data from each variable was analyzed using analysis of variance, and continued with the BNJ test at a significance level of 0,05. The results of the research showed that the type of basic media had no significant effect on all variables. Concentration 2 mg/L of BA gave the highest response to the variables of percentage of somatic embryo formation, number of SE per explant and number of shoots per clump.
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Yusnita. 2010. Perbanyakan In Vitro Tanaman Anggrek. Universitas Lampung. Bandar Lampung. 128 hlm.
Yusnita. 2015. Kultur Jaringan Tanaman: Sebagai Teknik Penting Bioteknologi Untuk Menunjang Pembangunan Pertanian. Aura. Bandar Lampung. 86 hlm.
Boldaji, H. N., Dylami, S. D., Aliniaeifard, S., dan Norouzi, M. 2021. Efficient method for direct embryogenesis in phalaenopsis orchid. International Journal of Horticultural Science and Technology, 9(2): 37–50.
Hapsoro, D. dan Yusnita. 2018. Kultur Jaringan “Teori dan Praktikâ€. Andi. Yogyakarta. 167 hlm.
Hapsoro, D. dan Yusnita. 2022. Embriogenesis Somatik In Vitro Untuk Perbanyakan Klonal dan Pemuliaan Tanamanâ€. Aura. Yogyakarta. 65 hlm.
Juntada, K., Taboonmee, S., Meetum, P., Poomjae, S., dan Chiangmai, P. N. 2015. Somatic Embryogenesis Induction from Protocorm-like Bodies and Leaf Segments of Dendrobium Sonia “Earsakul.â€. Silpakorn U Science & Tech J, 9(2): 9–19.
Kasi, P.D., dan Semiarti, E. 2017. Pengaruh thidiazuron dan naphtaleneacetic acid untuk induksi embriogenesis somatik dari daun anggrek Phalaenopsis “Sogo Vivienâ€. Dinamika, 7(1):31-40.
Lee, P. L., dan Chen, J. T. 2014. Plant regeneration via callus culture and subsequent in vitro flowering of Dendrobium huoshanense. Acta Physiologiae Plantarum, 36(10): 2619–2625.
Mayer, J.L.S., Stancato, G.C., dan Appezzato-Da-Glo´ria, B. 2010. Direct regeneration of protocorm-like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae). Plant Cell, Tissue and Organ Culture, 103: 411-416.
Manokari, M., Latha, R., Priyadharshini, S., Jogam, P., dan Shekhawat, M. S. 2020. Short‑term cold storage of encapsulated somatic embryos and retrieval of plantlets in grey orchid (Vanda tessellata (Roxb.) Hook. ex G.Don). Plant Cell, Tissue and Organ Culture, 144: 171-183.
Manokari, M., Priyadharshini, S., dan Shekhawat, M. S. 2021. Direct somatic embryogenesis using leaf explants and shortterm storage of synseeds in Spathoglottis plicata Blume. Plant Cell, Tissue and Organ Culture, 145(2): 321–331.
Melisa, A.O. 2011. Pemberian Kombinasi 2,4-D dan Kinetin Terhadap Induksi Kalus dan Protocorm Like Bodies (PLB) Anggrek Grammatophyllum scriptum Secara In Vitro. Skripsi. Jurusan Biologi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Sebelas Maret. Surakarta.
Minh, V. T. 2019. micropropagation of Rhynchostylis gigantea orchid by somatic embryogenic cultures. CBU International Conference Proceedings, 7: 969–974.
Mose, W., Daryono, B. S., Indrianto, A., Purwantoro, A., dan Semiarti, E. 2020. Direct Somatic Embryogenesis and Regeneration of an Indonesian orchid Phalaenopsis amabilis (L.) Blume under a Variety of Plant Growth Regulators, Light Regime, and Organic Substances. Jordan Journal of Biological Sciences, 13(4): 509-518.
Naing, A.H., Chung, J.D., dan Lim, K.B. 2011. Plant regeneration through indirect somatic embryogenesis in Coelogyne cristata orchid. American Journal of Plant Sciences, 2: 262-267
Naranjo, E.J., Fernandez Betin, O., Urrea Trujillo, A.I., Callejas Posada, R., dan Atehortúa Garcés, L. 2016. Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis. Acta Biol Colomb, 21: 87-98.
Pathak, P., Verma, S., Prakash, A., dan Mahant, K.C. 2017. Regeneration competence of an ornamentally important epiphytic orchid, Rhynchostylis gigantea (lindl.) ridl. through leaf segments: a study in vitro. J. Orchid Soc. India, 31: 97-101.
Purwanto, A.W. 2016. Anggrek: Budidaya dan Perbanyakan. LPPM UPN Veteran Yogyakarta Press. Yogyakarta. 120 hlm.
Pyati, A. N. 2022. In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis. Plant Tissue Cult. & Biotech, 32(1): 53-66.
Rachmawati, F., Permanik, D., Mayang, R. B., dan Winarto, B. 2019. Protokol Perbanyakan Masal Dendrobium ‘Balithi CF22-58’ secara In Vitro Melalui Embriogenesis Somatik Tidak Langsung. Jurnal Hortikultura, 29(2): 137-146.
Sujjaritthurakarn, P., dan Kanchanapoom, K. 2011. Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron. Not SSci Biol, 3(4): 88-92
Widiastoety, D., Solvia, N., dan Soedarjo, M. 2010. Potensi Anggrek Dendrobium Dalam Meningkatkan Variasi Dan Kualitas Anggrek Bunga Potong. Jurnal Litbang Pertanian, 29(3): 101-106.
Yusnita. 2010. Perbanyakan In Vitro Tanaman Anggrek. Universitas Lampung. Bandar Lampung. 128 hlm.
Yusnita. 2015. Kultur Jaringan Tanaman: Sebagai Teknik Penting Bioteknologi Untuk Menunjang Pembangunan Pertanian. Aura. Bandar Lampung. 86 hlm.
Published
2024-05-13
How to Cite
Hamiranti, R. (2024). Mass Propagation on Dendrobium Orchid ‘Gradita 31’ Through Somatic Embryogenesis. Savana Cendana, 9(2), 55-58. https://doi.org/https://doi.org/10.32938/sc.v9i2.2383
Section
Original research article
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