The Activity of Tannin Acyl Hydrolase Enzyme at Optimum Condition of Lactobacillus Plantarum Fermentation using Canna Flour (Canna edulis Kerr) as Substrate

  • Azor Yulianus Tefa Faculty of Agriculture, The University of Timor
  • Marselina Theresia Djue Tea Faculty of Agriculture, The University of Timor
Keywords: Canna, Lactobacillus Plantarum, Tannin Acyl Hydrolase

Abstract

This study aimed to determine the activity of tannin acyl hydrolase enzyme produced under the optimum conditions of Lactobacillus plantarum fermentation using canna flour (Canna edulis Kerr) flour as substrate. The research process began with the production of tannin acyl hydrolase enzyme, then proceeded with the isolation of the crude enzyme and then the activity of the tannin acyl hydrolase enzyme were measured. The variations of inoculum volume in this study were 1 mL, 3 mL, and 5 mL, with 2 grams of substrate. The variation of pH used when testing the enzyme activity was pH 4, pH 5, pH 6, and pH 7, the temperature variations used were 35°C, 40°C, 45°C, and 50°C, while the incubation time variations were 12 hours, 24 hours, 36 hours, and 48 hours. The results showed that the highest activity of tannin acyl hydrolase enzyme was obtained at 1.13 U/mL at optimum pH of 6, 0.88 U/mL at optimum temperature of 45°C, and 0.89 U/mL for optimum incubation time of 36 hours.

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Published
2022-10-31
How to Cite
Tefa, A., & Tea, M. (2022). The Activity of Tannin Acyl Hydrolase Enzyme at Optimum Condition of Lactobacillus Plantarum Fermentation using Canna Flour (Canna edulis Kerr) as Substrate. Savana Cendana, 7(04), 59-60. https://doi.org/https://doi.org/10.32938/sc.v7i04.1932
Section
Original research article