Use of probiotic bacteria to suppress Colletotrichum acutatum infections and improve chilli seeds (Capsicum annuum L.) quality during storage

  • Anna Tefa Faculty of Agriculture, Timor University, Kefamenanu, Indonesia.
  • Eny Widajati Departement of Agronomy and Horticulture, Bogor Agricultural University, Bogor, Indonesia.
  • Muhamad Syukur Departement of Agronomy and Horticulture, Bogor Agricultural University, Bogor, Indonesia.
  • Giyanto Giyanto Departement of Plant Protection, Bogor Agricultural University, Bogor, Indonesia.
Keywords: Chilli, Anthracnose, Bacillus sp., Pseudomonas sp., Actinomycetes sp, Seed coating, Storage period

Abstract

This study aimed to obtain probiotic bacteria  that are antagonistic bacteria to Colletotrichum acutatum and to improve chilli seeds quality. The purpose of this study was to obtain probiotic bacteria antagonistic to C. acutatum and prevent seed-borne diseases. The layout of the experiment arranged in completely randomized design factorial design with two factors. The first factor is the seed coating that consists of 6 levels i.e control / without coating (c0), coating without bacteria (c1), coating Bacillus sp. (c2), coating Pseudomonas sp. (c3), coating Actinomycetes sp. (c4) and fungicidal coating (c5). The second factor is the storage period is 0, 1, 2, 3, 4 and 5 months. The results showed that three bacteria that are antagonistic to C. acutatum, i.e.Actinomycetes sp. (ATS6) having inhibition percentage of56, 8%, Bacillus sp. (B48) having inhibition percentage 56,7% and Pseudomonas sp. (P12) having inhibition percentage 46,7%. Application of probiotic bacteria increased viability and the health of chilli seed for storage period of 5 months, seen from maximum growth potential 80-84%, germination capacity 76-78,7%, infected seeds in coating of Actinomycetes sp., 2,6% and Bacillus sp., 6,7%.Bacillus sp. contained 91,8 ppm indole acetic acid (IAA) and 103,2 ppm giberelins (GA3), Actinomycetes sp. contained 89,6 ppm IAA and 92,5 ppm giberelin and Pseudomonas sp. contained 68,9 ppm IAA and 69,2 ppm giberelin. 

Downloads

Download data is not yet available.

References

Asnawi, Iswati, R, Hiasinta&Motulo FJ 2012, Eksplorasi agens biokontrol Phytopthora palmivora penyebab penyakit gugur buah kelapa, JATT.,vol. 1, no. 2, hlm. 61-66.

Asian Vegetable Research Development Centre 2004, Fact Sheet, AVRDC publication 04-174, diunduh 14 September 2014, .

Feliatra, Fitria, Y & Nursyirwani 2012. Antagonis bakteri probiotik yang diisolasi dari usus dan lambung ikan kerapu bebek (Cromileptes altivelis) terhadap bakteri patogen, Jurnal Perikanan dan Kelautan.,vol. 17 no.1, hlm.16-25.

Food Agriculture Organization/World Health Organization 2001, Report of a joint FAO/WHO expert consultation on evaluation og health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, diunduh 15 September 2014.

Gunawan, OS 2006, Mikroba antagonis untuk pengendalian penyakit antraknos pada cabai besar, J. Hort., vol. 16 no. 2, hlm. 151-155.

Handoko, A, Abadi, AL &Aini, LQ 2014, Karakterisasi penyakit penting pada pembibitan tanaman Durian di Desa Plangkrongan, Kabupaten Magetan dan pengendalian dengan bakteri antagonis secara in vitro, Jurnal HPT., vol. 2, no. 2, hlm. 15-22.

Ibrahim, A, Ilyas, S & Manohara, D 2014, Perlakuan benih cabai (Capsicum annuum,L) dengan Rhizobacteri untuk mengendalikan Phytophthoracapsici, meningkatkan vigor benih dan pertumbuhan tanaman, Bul. Agrohorti.,vol.2, no. 1, hlm. 22-30.

Mattjik, AA, & Sumertajaya, IM 2006. Perancangan percobaan. IPBPres, Bogor.

Miransari, M & Smith, D 2009, Rhizobial Lipo-Chitooligosaccharides and Gibberellins enhance Barley (Hordeum vulgare L.) seed germination, Biotechnology., vol. 8, no. 2, hlm. 270-275.

Muchtar, SD, Widajati, E & Giyanto 2014, Pelapisan benih menggunakan bakteri probiotik untuk mempertahankkan viabilitas benih jagung manis (Zea mays saccharata Sturt.) selama penyimpanan, Bul. Agrohorti.,vol.1 no.4, hlm. 26-33.

Mugnisjah, WQ & Setiawan, A 2004, Produksi Benih. Bumi Aksara, Jakarta.

Muharni dan Widjajanti, H 2011, Skrining bakteri kitinolitik antagonis terhadap pertumbuhan jamur akar putih (Rigidopones lignosus) dari rizozfer tanaman karet, Jurnal Penelitian Sains., vol. 14, no.1, hlm. 14112-56.

Nurhayati 2011, Efektivitas ekstrak daun siri terhadap infeksi Colletotrichum capsici pada buah cabai, Dharmapala., vol. 3, no. 2,hlm. 54-59.

Nurkanto, A, Julistiono, H, Agusta, A & Sjamsuridzal, W 2012, Screening antimikroba activity of Actinomycetesisolated from Raja Ampat, West Papua, Indonesia, Makalah Journal of Science.,vol. 16, no. 1, hlm. 21-26.

Novina, D, Suryanto, D & Elimasni 2012, Uji potensi bakteri kitinolitik dalam menghambat pertumbuhan Rhizoctonia solani penyebab rebah kecambah pada kentang varietas granola, Saintia Biologi., vol. 1, no. 1, hlm. 1-7.

Sallytha, AAM, Addy, HS & Mihardjo,PA 2014, Penghambatan Actinomycetes Erwinia carotovora subsp. Carotovora secara in vitro, Berkala Ilmiah Pertanian.,vol.1, no.4,hlm.70-72.

Setiyowati, H, Surahman, M & Wiyono S, 2007, Pengaruh seed coating dengan fungisida benomil dan tepung curcuma terhadap patogen antraknosa terbawa benih dan viabilitas benih cabai besar (Capsicum annuum L), Buletin Agronomi., vol.35., no.3, hlm. 176-182.

Shahab, S, Ahmad, N & Khan, NS 2009, Indole acetic acid production and enhanced plant growth promotion by indigenous PSBs, African Journal of Agricultural Research., vol.4, no.11, hlm.1312-1316.

Siregar, AN, Ilyas, S, Fardiaz, D, Murniati, E & Wiyono, S 2007, Penggunaan agens biokontrol Bacillus polymixa dan Trichoderma harzianus untuk peningkatan mutu benih cabai dan pengendalian penyakit antraknosa, Jurnal Penyuluhan Pertanian., vol. 2, no. 2, hlm. 105-114.

Shrivastava, S, D’Souza SF, Desai PD 2008, Production of indole-3-acetic acid by immobilized Actinomycetes (Kitasatospora sp.) for soil applications. Current Science, vol. 94, no. 12, hlm. 1595-1604.

Syarif, R, Egad, L & Nurwitri, CC 2003. Mikotoksin bahan pangan, Institut Pertanian Bogor Press, Bogor.

Syukur, M, Sujiprihati S, Koswara J & Widodo 2007, Pewarisan ketahanan cabai (Capsicum annuum L) terhadap anthraknosa yang disebabkan oleh Colletotrichum acutatum, Bul. Agron.,vol. 35, no. 2, hlm. 112-117.

Wilia, W, Widodo & Wiyono, S 2012, Potensi khamir untuk mengendalikan penyakit anthraknosa (Colletotrichum acutatum L.) pada tanaman cabai, Online Journal UNJA., vol.1, no.4,hlm. 65-72.

Yus, IDM, Rahardjo, BT & Himawan, T 2014, Pengaruh aplikasi bakteri Pseudomonas fluorescens dan Bacillus subtilis terhadap mortalitas nematoda puru akar (Meloidogyne javanica) di Laboratorium, Jurnal HPT., Vol.2,no. 3, hlm.9-17.
Published
2016-01-15
How to Cite
Tefa, A., Widajati, E., Syukur, M., & Giyanto, G. (2016). Use of probiotic bacteria to suppress Colletotrichum acutatum infections and improve chilli seeds (Capsicum annuum L.) quality during storage. Savana Cendana, 1(01), 38-42. https://doi.org/https://doi.org/10.32938/sc.v1i01.10
Section
Original research article