Bioethanol Production from Dryland Sorghum Pulp with Preliminary Treatment of Microwave Irradiation

  • Sefrinus MD Kolo Universitas Timor
  • Yuni Sine
Keywords: Sorghum, Hydrolysis, Microwave, Reducing Sugar, Bioethanol


One potential ingredient that has a high starch content is sorghum (Sorghum bicolor L.). Sorghum (Sorghum bicolor L.) has broad agroecological adaptability, is resistant to drought, is resistant to pests and diseases compared to other plants. The high carbohydrate content (amylose and amylopectin) of around 73 g / 100 g material from sorghum is very possible to be converted into ethanol. This study aims to determine the optimum reducing sugar concentration before and after the initial treatment by microwave and to get bioethanol with the highest levels and percent yield. A total of 10 grams of sorghum powder were suspended with an aqueous solution of H2SO4 1%, 2% and 3% as much as 250 mL and then heated using microwave irradiation at temperatures of 100, 150, 200 and 250 oC for 20, 30, and 40 minutes. The liquid fraction of hydrolysis results was analyzed by reducing sugar content by the DNS method using a UV-Vis spectrophotometer. The results of the DNS (Dinitrosalisilat) analysis showed that reducing sugar levels increased with increasing hydrolysis temperatures at 100 0C to 150 0C (2.8 - 4.4 mg / L) and decreased levels at temperatures 200 and 250 0C. The results of hydrolysis optimization with variations in concentration over 30 and 40 minutes obtained reducing sugar levels increase with increasing acid concentration at the time of hydrolysis 30 minutes ie 2.0-8.5 mg / L and experienced a significant increase when extending the hydrolysis time 40 minutes ie 19, 1-42.7 mg / L. Reducing sugar concentration for ethanol production using a temperature of 150 0C with an acid concentration of 2% at the time of hydrolysis 40 minutes which is equal to 34.3 mg / L. Bioethanol Production from Dry Soil Sorghum Waste with Initial Treatment of Microwave Irradiation.


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Bintang, M. 2010. Teknik Penelitian Biokimia. Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB. Bogor. Indonesia.

Kolo, S.M.D; Wahyuningrum, D; Hertadi, R. (2016). Hidrolisis Rumput Gajah (Pennisetum Purpureum) Dengan Microwave Assisted Organic Synthesis (Maos) Untuk Produksi Xilooligosakarida Pembentuk Bioetanol. In R. A. F.
Siburian (Ed.), Seminar Nasional Laboratorium Riset Terpadu Undana (pp. 46–50). Kupang: Undana Press.

Kolo, S. M. D., Edi, E., (2018). Hidrolisis Ampas Biji Sorgum dengan Microwave untuk Produksi Gula Pereduksi sebagai Bahan Baku Bioetanol. JSLK, 1 (2) 22-23.

Kumar, P., Barret D.M., Delwiche, J., Stroeve, P. (2009): Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production. Journal of American Chemical Society 48(8), pp 3713–3729.

Kurniawan, W. (2014). Potensi sorgum numbu, cty-33, dan bmr sebagai pakan pada beberapa level pupuk kandang di tanah sedimentasi ultisol widhi kurniawan.

Leo M.L & T. Fidel, N. (2010). H A N D B O O K O F Dairy Foods Analysis. Retrieved from

Nikolić, S., Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Pejin, D., & Pejin, J. (2010). Improvement of bioethanol production from corn by ultrasound and microwave pretreatments. Chemical Engineering Transactions, 21, 1327–1332.

Wang, H., Mirela L, Maxim., Gabriela Gurau., Robin D. Rogers. (2013). Microwave-Assisted Dissolution and Delignification of Wood in 1-ethyl-3-methylimidazolium acetate. Bioresoruce Technology. 136: 739-742.

Xiang, Q., Lee, Y. Y., Petterson, P. O., & Torget, R. W. (2003). Heterogeneous aspects of acid hydrolysis of α-cellulose. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 107(1–3), 505–514.
How to Cite
Kolo, S., & Sine, Y. (2019). Bioethanol Production from Dryland Sorghum Pulp with Preliminary Treatment of Microwave Irradiation. Jurnal Saintek Lahan Kering, 2(2), 39-40. Retrieved from
Original research article