SUBSTRATE AND TEMPERATURE OF CRUDE EXTRACT LIPASE Aspergillus niger M1407

  • Amelia M Salmon Universitas Kristen Artha Wacana
  • Mellissa E. S. Ledo Universitas Kristen Artha Wacana
  • Merpiseldin Nitsae Universitas Kristen Artha Wacana
Keywords: Aspergillus niger M1407, characterization, substrate, crude extract lipase

Abstract

Isolation of crude extract lipase Aspergillus niger M1407 using kesambi seeds flour medium through solid state fermentation was carried for substrate and temperature characterization fo determenie optimal condition enzyme substarate reaction. The aim of this study are determenie the substrate and temperature profile crude extract lipase Aspergillus niger M1407. This research was conducted in the laboratory (microbiology and chemistry) of the Biology Education Study Program at Artha Wacana Christian University, Kupang, in January 2019 untel March 2019. The method used in this study divided into four stages, namely  medium preparation for Aspergillus niger M1407 lipase; Solid-State Fermentation medium for 7 days; substrate characterization using oleic acid + ethanol in isooktan and oleic acid + methanol in isooktan and temperature characterization was done at 20oC, 30oC, 40oC, 50oC, and 60oC. Analysis of  crude extract lipase Aspergillus niger M1407 activity using the titration method. Data obtained in quantitative descriptive analysis. The results of this study indicate that the crude extract lipase Aspergillus niger M1407. crude enzyme lipase Aspergillus niger M1407  activity is better on oleic acid methanol + substrate which has 200 U / mL activity while temperature characterization show best activity at 40oC with 580 u / mL lipase activity.

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References

Aziz, P. 2012. Enzim dan Faktor-faktor yang mempengaruhi Laju Reaksi Enzim. Addtion material for FIK Biochemical Experiment Class. Diakses tanggal 15 April 2012.

Gandjar, I., Robert, A.S., Karin van den, T. V ., Ariyati, O., dan Iman, S. 1999.Mengenali Kapang Tropik Umum. Yayasan Obor Indonesia. Jakarta.

Gubitz, G.M., M.Mittelbach dan M.Trabi. 1999. Exploitation of the tropical seed plant Jatropha curcas L., Bioresource Technology.

Hidayat, C. Darmasiwi, S. Nurikasari, M. 2015. Characterization of AspergillusNiger 65i6 lipase fromsolid-state fermentation using Jatrophaseed cake medium.Insdonesian journal of Biotechnology. Vol. (2) pp. 108-116.Yogyakarta.

Ledo, S.E. Mellissa, Dima, R. Yemima, Nope, V.V. Jumita. 2016. Aktivitas Lipolitik Aspergillus niger dan penicillum sp Indigenius yang diisolasi dari Biji Kesambi (schleichera oleosa). Prosiding seminar nasional sainstek ke-3.28-29 Oktober 2016. UNDANA. Kupang.

Lestari, P.,Handayani, S.N. dan Oedjijono. 2009. Sifat-sifat Biokimiawi Ekstrak Kasar Lipase Ekstraseluler Dari Bakteri Azospirillum sp. JG3. Program Studi Kimia, Jurusan MIPA Fakultas Sains dan Teknik UNSOED, Purwokerto Fakultas Biologi UNSOED. Purwokerto.

Masfufatun. 2010. Isolasi dan Karakterisasi Enzim Selulase.(Skripsi) Fakultas Kedokteran Universitas Wijaya Kusuma. Surabaya.

Murni,W. Sri, Kholisoh, D. Siti, L.D. Tanti, M. E. Petrissia. 2011. Produksi, Karakterisasi, dan Isolasi Lipase dari Aspergillus niger. Prosiding seminar nasionl teknik kimia, pengembangan teknologi kimia untuk pengolahan Sumber Daya Alam Indonesia. Program Studi Kimia Fakultas Teknologi Industri. ISSN 1693-4393.

Nurosid.,Oedjijono.danLestari,P.2008.Kemampuan Azospirillum sp.JG3 Dalam Menghasilkan Lipase Pada Medium Campuran Dedak Dan Onggok Dengan Waktu Inkubasi Berbeda.Departement of Microbiologi, Biology Faculty Jenderal Soedirman University.Purwokerto.

Rahman, A. 1989. Teknologi fermentasi industrial. Bogor. Institut Pertanian Bogor. Winarno, F.G. 1989. Enzim Pangan. Garamedia Press. Jakarta.
Published
2020-06-13
How to Cite
Salmon, A., Ledo, M., & Nitsae, M. (2020). SUBSTRATE AND TEMPERATURE OF CRUDE EXTRACT LIPASE Aspergillus niger M1407. Jurnal Saintek Lahan Kering, 3(1), 13-15. https://doi.org/https://doi.org/10.32938/slk.v3i1.1038
Section
Original research article