DIAZINON ANALYSIS ON APPLE FARMLAND IN BUMIAJI USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Abstract
A method for the determination of diazinon in soil samples has been developed. The analyte was extracted with acetonitrile from farmland soil sample. Determination and quantification of diazinon were perfomed by high perfomance liquid chromatography (HPLC) with an UV detector. Optimum conditions for diazinon analysis were eluent composition of acetonitrile:water ratio of 60:40, 0.4 mL/min of flow rate, and 220 nm of wavelength. Under the optimum conditions, diazinon linearity was between 1 and 25 mgL-1 with R2 of 0.9976, 1.19 mgL-1 LOD, and 3.98 mgL-1 LOQ. When the method was applied to the soil sample, both pesticides showed acceptable recoveries for real sample of more than 85%: thus, the developed method meets the validation requirement. Under this developed method, the concentrations of diazinon in the soil samples were below the LOD and LOQ (0.577 mgL-1). Therefore, it can be concluded that the soil samples used in this study did not contain diazinon.
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References
Griffith, W. C., Vigoren, E. M., Smith, M. N., Thompson, B., Coronado, G. D., Elaine, M., Sciences, O. H., Communication, R., Program, P., & Hutchinson, F. 2019. Application of Improved Approach to Evaluate a Community Intervention to Reduce Exposure of Young Children Living in Farmworker Households to Organophosphate Pesticides. J Expo Sci Environ Epidemiol, 29(3), 358–365. https://doi.org/10.1038/s41370-018-0028-y.Application.
Harmita. 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117–135.
Hartanti, E., Mahatmanti, F., & Susatyo, E. (2012). Sintesis Kitosan_Bentonit serta Aplikasinya sebagai Penurun Kadar Insektisida Jenis Diazinon. Indonesian Journal of Chemichal Science, 1, 111–115.
Indrianingsih, A., Nisa, K., Wahono, S., Taufika, V., Rosdya, Damayanti, E., & Maryana, R. 2007. Analisis Residu Pestisida dalam Tanah dan Umbi Bawang Merah di Lahan Pasir Sanden , Bantul , Yogyakarta dengan Kromatografi Gas. Seminar Nasional Rekayasa Kimia Dan Proses 2007 ISSN: 1411-4216, 181–185.
Mulder, T. A., Dries, M. A. Van Den, Korevaar, T. I. M., & Ferguson, K. K. 2019. Organophosphate Pesticides Exposure in Pregnant Women and Maternal and Cord Blood Thyroid Hormone Concentrations. Environment International, 132, 105124. https://doi.org/10.1016/j.envint.2019.105124
Nurhamidah. 2005. Penentuan Kondisi Optimum HPLC untuk Pemisahan Residu Pestisida Imidakloprid, Profenofos, dan Deltametrin pada Cabai (Capsicumannum). Jurnal Ilmu-Ilmu Pertanian Indonesia, 7(2), 87–93.
Pertanian Sehat. 2012. https://pertaniansehat.com/read/2012/08/09/pentingnya-analisis-residu-pestisida.html, diakses tanggal 13 Januari 2016.
Sánchez, M. E., Méndez, R., Gómez, X., & Martín-Villacorta, J. 2007. Determination of Diazinon and Fenitrothion in Environmental Water and Soil Samples by HPLC. Journal of Liquid Chromatography and Related Technologies, 26, 483–497. https://doi.org/10.1081/JLC-120017184
Sanchez-Brunete, C., Albero, B., & Tadeo, J. 2004. Multiresidue Determination of Pesticides in Soil by Gas Chromatography − Mass Spectrometry Detection. Journal of Agricultural and Food Chemistry, 52, 1445–1451.
Shemer, H., & Linden, K. G. 2006. Degradation By-product Formation and of Diazinon in Water During UV and UV/H2O2 Treatment. Journal of Hazardous Materials, 136(3), 553–559. https://doi.org/10.1016/j.jhazmat.2005.12.028
Syahbirini, G., & Pumama, H. 2001. Pesticide Residues in Three Kinds of Imported Fruits. Buletin Kimia, 1, 113–118.
Tang, J., Zhang, M., Cheng, G., & Lu, Y. 2009. Preparation and Evaluation of Griffith, W. C., Vigoren, E. M., Smith, M. N., Thompson, B., Coronado, G. D., Elaine, M., Sciences, O. H., Communication, R., Program, P., & Hutchinson, F. 2019. Application of Improved Approach to Evaluate a Community Intervention to Reduce Exposure of Young Children Living in Farmworker Households to Organophosphate Pesticides. J Expo Sci Environ Epidemiol, 29(3), 358–365. https://doi.org/10.1038/s41370-018-0028-y.Application
Harmita. 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117–135.
Hartanti, E., Mahatmanti, F., & Susatyo, E. (2012). Sintesis Kitosan_Bentonit serta Aplikasinya sebagai Penurun Kadar Insektisida Jenis Diazinon. Indonesian Journal of Chemichal Science, 1, 111–115.
Indrianingsih, A., Nisa, K., Wahono, S., Taufika, V., Rosdya, Damayanti, E., & Maryana, R. 2007. Analisis Residu Pestisida dalam Tanah dan Umbi Bawang Merah di Lahan Pasir Sanden , Bantul , Yogyakarta dengan Kromatografi Gas. Seminar Nasional Rekayasa Kimia Dan Proses 2007 ISSN: 1411-4216, 181–185.
Mulder, T. A., Dries, M. A. Van Den, Korevaar, T. I. M., & Ferguson, K. K. 2019. Organophosphate Pesticides Exposure in Pregnant Women and Maternal and Cord Blood Thyroid Hormone Concentrations. Environment International, 132, 105124. https://doi.org/10.1016/j.envint.2019.105124.
Nurhamidah. 2005. Penentuan Kondisi Optimum HPLC untuk Pemisahan Residu Pestisida Imidakloprid, Profenofos, dan Deltametrin pada Cabai (Capsicumannum). Jurnal Ilmu-Ilmu Pertanian Indonesia, 7(2), 87–93.
Pertanian Sehat. 2012. https://pertaniansehat.com/read/2012/08/09/pentingnya-analisis-residu-pestisida.html, diakses tanggal 13 Januari 2016.
Sánchez, M. E., Méndez, R., Gómez, X., & Martín-Villacorta, J. 2007. Determination of Diazinon and Fenitrothion in Environmental Water and Soil Samples by HPLC. Journal of Liquid Chromatography and Related Technologies, 26, 483–497. https://doi.org/10.1081/JLC-120017184
Sanchez-Brunete, C., Albero, B., & Tadeo, J. 2004. Multiresidue Determination of Pesticides in Soil by Gas Chromatography − Mass Spectrometry Detection. Journal of Agricultural and Food Chemistry, 52, 1445–1451.
Shemer, H., & Linden, K. G. 2006. Degradation By-product Formation and of Diazinon in Water During UV and UV/H2O2 Treatment. Journal of Hazardous Materials, 136(3), 553–559. https://doi.org/10.1016/j.jhazmat.2005.12.028
Syahbirini, G., & Pumama, H. 2001. Pesticide Residues in Three Kinds of Imported Fruits. Buletin Kimia, 1, 113–118.
Tang, J., Zhang, M., Cheng, G., & Lu, Y. 2009. Preparation and Evaluation of Sol-Gel Glass-Based Immunoaffinity Column and Their Potential Use in Determination of Diazinon in Water and Soil Samples with High Performance Liquid Chromatography. Analytical Letters, 42, 243–254. https://doi.org/10.1080/00032710802585709
Tette, P., Guidi, L., Gloria, M., & Fernandes, C. 2016. Talanta Pesticides in Honey: A Review on Chromatographic Analytical Methods. Talanta, 149, 124–141. https://doi.org/10.1016/j.talanta.2015.11.045
Harmita. 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117–135.
Hartanti, E., Mahatmanti, F., & Susatyo, E. (2012). Sintesis Kitosan_Bentonit serta Aplikasinya sebagai Penurun Kadar Insektisida Jenis Diazinon. Indonesian Journal of Chemichal Science, 1, 111–115.
Indrianingsih, A., Nisa, K., Wahono, S., Taufika, V., Rosdya, Damayanti, E., & Maryana, R. 2007. Analisis Residu Pestisida dalam Tanah dan Umbi Bawang Merah di Lahan Pasir Sanden , Bantul , Yogyakarta dengan Kromatografi Gas. Seminar Nasional Rekayasa Kimia Dan Proses 2007 ISSN: 1411-4216, 181–185.
Mulder, T. A., Dries, M. A. Van Den, Korevaar, T. I. M., & Ferguson, K. K. 2019. Organophosphate Pesticides Exposure in Pregnant Women and Maternal and Cord Blood Thyroid Hormone Concentrations. Environment International, 132, 105124. https://doi.org/10.1016/j.envint.2019.105124
Nurhamidah. 2005. Penentuan Kondisi Optimum HPLC untuk Pemisahan Residu Pestisida Imidakloprid, Profenofos, dan Deltametrin pada Cabai (Capsicumannum). Jurnal Ilmu-Ilmu Pertanian Indonesia, 7(2), 87–93.
Pertanian Sehat. 2012. https://pertaniansehat.com/read/2012/08/09/pentingnya-analisis-residu-pestisida.html, diakses tanggal 13 Januari 2016.
Sánchez, M. E., Méndez, R., Gómez, X., & Martín-Villacorta, J. 2007. Determination of Diazinon and Fenitrothion in Environmental Water and Soil Samples by HPLC. Journal of Liquid Chromatography and Related Technologies, 26, 483–497. https://doi.org/10.1081/JLC-120017184
Sanchez-Brunete, C., Albero, B., & Tadeo, J. 2004. Multiresidue Determination of Pesticides in Soil by Gas Chromatography − Mass Spectrometry Detection. Journal of Agricultural and Food Chemistry, 52, 1445–1451.
Shemer, H., & Linden, K. G. 2006. Degradation By-product Formation and of Diazinon in Water During UV and UV/H2O2 Treatment. Journal of Hazardous Materials, 136(3), 553–559. https://doi.org/10.1016/j.jhazmat.2005.12.028
Syahbirini, G., & Pumama, H. 2001. Pesticide Residues in Three Kinds of Imported Fruits. Buletin Kimia, 1, 113–118.
Tang, J., Zhang, M., Cheng, G., & Lu, Y. 2009. Preparation and Evaluation of Griffith, W. C., Vigoren, E. M., Smith, M. N., Thompson, B., Coronado, G. D., Elaine, M., Sciences, O. H., Communication, R., Program, P., & Hutchinson, F. 2019. Application of Improved Approach to Evaluate a Community Intervention to Reduce Exposure of Young Children Living in Farmworker Households to Organophosphate Pesticides. J Expo Sci Environ Epidemiol, 29(3), 358–365. https://doi.org/10.1038/s41370-018-0028-y.Application
Harmita. 2004. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117–135.
Hartanti, E., Mahatmanti, F., & Susatyo, E. (2012). Sintesis Kitosan_Bentonit serta Aplikasinya sebagai Penurun Kadar Insektisida Jenis Diazinon. Indonesian Journal of Chemichal Science, 1, 111–115.
Indrianingsih, A., Nisa, K., Wahono, S., Taufika, V., Rosdya, Damayanti, E., & Maryana, R. 2007. Analisis Residu Pestisida dalam Tanah dan Umbi Bawang Merah di Lahan Pasir Sanden , Bantul , Yogyakarta dengan Kromatografi Gas. Seminar Nasional Rekayasa Kimia Dan Proses 2007 ISSN: 1411-4216, 181–185.
Mulder, T. A., Dries, M. A. Van Den, Korevaar, T. I. M., & Ferguson, K. K. 2019. Organophosphate Pesticides Exposure in Pregnant Women and Maternal and Cord Blood Thyroid Hormone Concentrations. Environment International, 132, 105124. https://doi.org/10.1016/j.envint.2019.105124.
Nurhamidah. 2005. Penentuan Kondisi Optimum HPLC untuk Pemisahan Residu Pestisida Imidakloprid, Profenofos, dan Deltametrin pada Cabai (Capsicumannum). Jurnal Ilmu-Ilmu Pertanian Indonesia, 7(2), 87–93.
Pertanian Sehat. 2012. https://pertaniansehat.com/read/2012/08/09/pentingnya-analisis-residu-pestisida.html, diakses tanggal 13 Januari 2016.
Sánchez, M. E., Méndez, R., Gómez, X., & Martín-Villacorta, J. 2007. Determination of Diazinon and Fenitrothion in Environmental Water and Soil Samples by HPLC. Journal of Liquid Chromatography and Related Technologies, 26, 483–497. https://doi.org/10.1081/JLC-120017184
Sanchez-Brunete, C., Albero, B., & Tadeo, J. 2004. Multiresidue Determination of Pesticides in Soil by Gas Chromatography − Mass Spectrometry Detection. Journal of Agricultural and Food Chemistry, 52, 1445–1451.
Shemer, H., & Linden, K. G. 2006. Degradation By-product Formation and of Diazinon in Water During UV and UV/H2O2 Treatment. Journal of Hazardous Materials, 136(3), 553–559. https://doi.org/10.1016/j.jhazmat.2005.12.028
Syahbirini, G., & Pumama, H. 2001. Pesticide Residues in Three Kinds of Imported Fruits. Buletin Kimia, 1, 113–118.
Tang, J., Zhang, M., Cheng, G., & Lu, Y. 2009. Preparation and Evaluation of Sol-Gel Glass-Based Immunoaffinity Column and Their Potential Use in Determination of Diazinon in Water and Soil Samples with High Performance Liquid Chromatography. Analytical Letters, 42, 243–254. https://doi.org/10.1080/00032710802585709
Tette, P., Guidi, L., Gloria, M., & Fernandes, C. 2016. Talanta Pesticides in Honey: A Review on Chromatographic Analytical Methods. Talanta, 149, 124–141. https://doi.org/10.1016/j.talanta.2015.11.045
Published
2020-06-13
How to Cite
Tea, M. T. (2020). DIAZINON ANALYSIS ON APPLE FARMLAND IN BUMIAJI USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Jurnal Saintek Lahan Kering, 3(1), 26-28. https://doi.org/https://doi.org/10.32938/slk.v3i1.1021
Section
Original research article
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